A sixth defeat in seven matches has dropped Winchester into trouble in South League Premier Division.
Oxford University's 3-2 win at Kings' has dropped Winchester into third from bottom spot ahead of demanding pre-Christmas games against London Edwardians and table topping Spencer.
Jack Culmer and Kieran Molloy scored for Winchester, who lie three points ahead of Henley, who trimmed Milton Keynes 2-0.
Winchester seconds lost 4-1 at Wimborne Wayfarers, with Stu Swift scoring the consolation goal.
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